Composite
Part:BBa_K1748007
Designed by: Thomas Bridge Group: iGEM15_Westminster (2015-09-17)
MtrC-His
Part BBa_K1748001 (MtrC) with added His-10 tag. Made using RPD standard and converted into BioBrick format, contains medium strength promoter, medium strength Rbs, coding sequence, cleavable protein linker, His-10 tag.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 29
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 12
Illegal BsaI.rc site found at 2160
[edit]
Categories
Parameters
None |